分类
DNA 纳米颗粒 蛋白质
RNA 3' 末端分析
电泳
表观转录组
miRNA 干扰
miRNA与mRNA 的相互作用
mRNA 转译
qRT-PCR
返转录
RNA 加帽
RNA 降解
RNA 检测 RNA 提取
RNA 干扰
RNA 标记
RNA定位
RNA 修饰
RNA 纯化 RNA 测序
RNA 剪接
RNA 结构
RNA 合成
RNA-蛋白质相互作用
转录
转染
Northern Blot with IR Fluorescent Probes: Strategies for Probe Preparation
利用多种标记方法进行IR标记探针的Northern杂交
Northern blot is a molecular biology technique that can detect, quantify, and determine the molecular weight of RNA. Recently, we published a protocol utilizing near-infrared (IR) fluorescent probes in Northern blot (irNorthern). Our method is as sensitive as other non-radioactive methods but is more straightforward and versatile. Additionally, we found that IR-labeled probes can be used to multiplex or detect different species of RNA at the same time. Here we describe three methods for generating an IR-labeled probe as well as how to perform irNorthern blot. In conclusion, our irNorthern protocol offers a convenient method for RNA detection.